Essay On Immunocastration In Milk - 747 Words

In addition, consistent with the low density of spermatogonia, spermatids and seminiferous tubules observed in testicles, the mRNA expressions of LH-ÃŽ ² and FSH-ÃŽ ² in the pituitary gland were both significantly decreased after immunocastration in the treated rams. The result is in agreement with the findings of [Wei et al., 2011] in rabbit and [Fang et al., 2010] in boar that GNRHR mRNA expression in the pituitary was significantly decreased in immunocastrated animals. Furthermore, [Sakurai et al., 1992] reported that there was a reduction of GNRHR mRNA in the pituitary and protein concentration after passive immunocastration of male sheep. Another group showed that the amount of GNRHR was significantly lower in the sheep that were immunized†¦show more content†¦In general, oral immunization with active kisspeptin DNA vaccine plays a significant role in suppressing GNRH secretion in the hypothalamus, GNRHR in the pituitary, and negative regulation of the secretion of ster oid hormones. Leydig cells have essential roles in the maintenance of steroidogenesis and spermatogenesis in the testes. The failures of both processes are attributed to Leydig cell dysfunction. In the current research, expression of testicular LH receptor and FSH receptor mRNAs was significantly lower in the treated ram lambs (P 0.05), implying that testicular responsiveness to LH stimulation was impaired. Indeed, active immunization against GNRH in boars significantly reduced testicular LH receptor and testosterone concentrations following treatment with exogenous LH [Wei et al. 2011; Wei et al., 2012]. Moreover, expression of testicular LHR mRNA was significantly decreased, which further confirmed that the functions of Leydig within testes were severely impaired. In addition, it is well known that FSH plays a role in spermatogenesis. However, the expression levels of FSHR mRNA in the testes of treated rams and control rams were not siginificantly different, demonstrating oral kisspeptin DNA im munization has no influence on